Biochemical Applications of Chromatography-HPLC

HPLC is a very common method for metabolomics analysis. With the invention of electrospray ionization, HPLC is coupled to mass spectroscopy. HPLC has lower chromatographic resolution, requires no derivation for polar molecules and separates molecules in the liquid phase. HPLC has the advantage of much wider range of analytes measurements with a higher sensitivity than gas chromatographic methods. Relevant to proteomics, due to the complex structure and nature of proteins, instrumentation and methods development for sample clean-up, pre-concentration, fractionation, chromatographic separation and detection becomes an immediate requirement for the identification of peptides and proteins. Latest techniques and equipment for separation and detection include nano-HPLC and multidimensional HPLC for protein and peptide separation. HPLC is considered as most reliable and most sensitive technique in genomics used to determine DNA methylation. The nucleosides and nucleotides of DNA are separated and quantified by HPLC-UV method. HPLC finds applications in glycomics and lipidomics where glycan part is cleaved either enzymatically or chemically from the target and subjected to analysis. HPLC has a wide application in lipidomics to separate lipids prior to mass spectrometry. Separation can be achieved by either reverse-phase (RP) HPLC or normal-phase (NP) HPLC.

  • Proteomics
  • Lipidomics
  • Biopharmaceutical data screening
  • Clinical Diagnosis
  • Food Technology
  • Nano Technology

Related Conference of Biochemical Applications of Chromatography-HPLC

September 25-26, 2017

5th International Conference on Current Trends in Mass Spectrometry and Chromatography


(10 Plenary Forums - 1Event)
Atlanta, Georgia, USA
October 02-04, 2017

2nd International Conference on Pharmaceutical Chemistry

Barcelona, Spain
(10 Plenary Forums-1Event)
November 02-03, 2017

18th World Conference and Exhibition on Analytical & Bioanalytical Chromatographic Techniques

Atlanta, Georgia, USA
November 15-16, 2017

2nd International Conference on Nuclear Chemistry


(10 Plenary Forums - 1Event)
Las Vegas, Nevada, USA
March 01 - 03, 2018

4th European Organic Chemistry Congress

London, UK
May 17-19, 2018

Global Experts Meeting on Chemistry

Singapore
23-24 July, 2018 Lisbon, Portugal

9th Global Chemistry Congress

8th European Chemistry Congress

June 21-23, 2018 Paris, France
November 05-06, 2018

International Conference on Catalysis and Pyrolysis 2018 (ICCP-2018)


(10 Plenary Forums - 1Event)
San Francisco, USA

Biochemical Applications of Chromatography-HPLC Conference Speakers

Recommended Sessions

Related Journals

Are you interested in