Rafal Gajos
Medical University of Lublin, Poland
Title: The orthogonal pressurized planar electrochromatography (OPPEC) - Its application to micro-preparative separation
Biography
Biography: Rafal Gajos
Abstract
Orthogonal pressurized planar electrochromatography (OPPEC) is a separation technique in which mixture components are separated in an adsorbent layer of a chromatographic plate. In such separation system a mobile phase solution flows through the adsorbent layer of the chromatographic plate in the defined direction and electric field is orthogonally applied to it. Therefore, the OPPEC is a combination of both chromatographic and electrophoretic techniques into a single separation technique, in which both processes proceed simultaneously and orthogonally. This technique can be applied to analytical and micro-preparative separations, along with a collection of separated fractions. When preparative mode is applied then the solutes can be separated inthe adsorbent layer in form of bands deviatedunder different angles from the direction of the mobile phase flow. In addition the separation system of the technique can be continuously fed with a sample mixture and separated components can be continuously collected at the outlet of the system. The last feature is especially attractive for preparative separations. In the presentation we would like to focus on the preparative mode of separation withthe OPPEC technique. Based on the obtained results we want to present the possibility of using our device to separate components of test dye mixture showing different deviation angle of their bands from the direction of the mobile phase flow. In addition, we intend to present a separation mode of solutes, which show the same deviation angle of their bands from the direction of the mobile phase flow.