Gaelle Coussot
Université de Montpellier, France
Title: Development of analytical fingerprints for the quality control of snake venoms raw substances
Biography
Biography: Gaelle Coussot
Abstract
Snake venoms have been of increasing interest in therapeutic research due to their rich composition in peptides and proteins. Drug formulations containing snake venom strains have already been commercialized mainly in homeopathy. Quality control (QC) of raw materials used in the composition of drugs is required for Good Manufacturing Practices. The high complexity as well as the qualitative and quantitative variations observed between animals of the same species renders snake venoms CQ difficult to implement. In this context, we have developed a fingerprint approach combining capillary electrophoresis and chemometrics for the QC of snake venom raw substances. CE is a particularly well suited technique for peptides/proteins separation. Batches of snake venom have been analyzed with many replicates. All results have been processed using several chemometrics approaches (baseline correction, signals alignment, automatic recognition of common peaks) to obtain a representative analytical trace (i.e. electropherogram) that can be used for the quality assessment of future production lots. The poster presents results obtained for Lachesis muta venom. Lachesis muta venom is widely used to treat among others thrombosis and neurological disorders.