Theme: Exhorting and Promulgating the Widespread Applications of Chromatography and Advancements in HPLC

HPLC Congress 2017

HPLC Congress 2017

Track 1:- Advances in Various Chromatographic Techniques

Chromatography basically is a method of separation of compounds from a mixture. The technique is both analytical and preparative and is employed widely in industries as well as in laboratories. Chemical analysis is mostly done all over the world with chromatography or any other various techniques related to chromatography. Chromatography is a physical technique and has a vast application in chemical field starting from basic organic chemistry to forensic science. Some common applications include lead in water, detection of cocaine in urine, PCB’s in fish and alcohol in blood. Various types of chromatography techniques are- paper chromatography, displacement chromatography, thin layer chromatography, supercritical chromatography, column fluid chromatography, gas chromatography, expanded bed adsorption chromatography, liquid chromatography in various combinations.The increasing demand for monoclonal antibodies is the main factor for chromatography resin market globally. Chromatographic resins are used in purification of proteins and separation of biomolecules in pharmaceuticals, food production, biotechnology, water and environmental analysis. Biotech and pharma industries are the largest user of chromatography resins followed by food production. North America has the highest market share of chromatography resins in terms of both consumption and revenue followed by Europe. The income from sales of resins in food production industry in North America is expected to record highest CAGR in the next five years. However, in Asian countries the chromatography resin consumption shows huge feedback to grow in the pharmaceutical sector. The raising market in emerging economies such as India, South Korea and China is expected to give a positive response in the chromatography resins market in the coming decade.

Column chromatography is a method used to purify individual chemical compounds from mixtures of compounds. It is has preparative applications on scales ranging from small scale to large scale production. Relatively low cost and disposability of the stationary phase are the main advantages of column chromatography.

Paper chromatography involves placing a small dot or line of sample solution onto a strip of polar cellulose chromatography paper. The paper is placed in a glass chamber with a shallow layer of solvent and is sealed. As the solvent moves through the paper, it comes in contact with the sample mixture, which starts to rise up the paper with the solvent.

Thin layer chromatography (TLC) is a mostly used technique which involves a stationary phase of a thin layer of adsorbent like alumina, silica gel, or cellulose on a flat, inert layer of substrate. Advantages of TLC are better separations, faster runs, and the choice of different adsorbents. Better quantification and resolution can be achieved with high-performance TLC.

Displacement chromatography is a preparative technique in which a sample is placed onto the head of the column and is then displaced by a solute that is more strongly adsorbed than the components of the original mixture. As a result the components are resolved into consecutive rectangular zones of highly concentrated pure substances rather than solvent-separated peaks. Higher product concentration and higher purity are obtained compared to other modes of chromatography.

Gas chromatography (GC) is commonly used in analytical chemistry for separating and analysing compounds that can be vaporized without decomposition. In this process, the mobile phase (or "moving phase") is a carrier gas; commonly an inert gas such as helium or an unreactive gas such as nitrogen is generally used. Stationary phase is a microscopic layer of liquid or polymer on an inert solid support, within a glass or metal tubing called a column and the instrument is called a gas chromatograph in which gas chromatography is being processed. The gaseous compounds being analysed interact with the walls of the column coated with a stationary phase.

Supercritical fluid chromatography (SFC) –in this technique the mobile phase is a fluid above and relatively close to its critical temperature and pressure. SFC mainly utilizes carbon dioxide as the mobile phase; in order to pressurize the chromatographic flow. Supercritical phase represents a state in which liquid and gas properties combine. Supercritical fluid chromatography is sometimes called as convergence chromatography. Supercritical fluid chromatography is a technique in which the mobile phase is a fluid operating at a relatively close critical temperature and pressure of the fluid.

Expanded Bed Adsorption (EBA) Chromatographic Separation is used for target protein from a raw feed stream when it passes through a chromatography column system containing adsorbent beads. Using this technique the unprocessed raw compound can be treated directly in the chromatographic column, avoiding clarification and pre-treatment steps.

Related Conferences of Advances in Various Chromatographic Techniques

International Conference and Exhibition on Analytical and Bio-analytical Techniques, September 29- October 1, 2016, Miami, USA; International Conference on Current Trends in Mass Spectrometry, May 9-11, 2016, Chicago, USA; World Chemistry Conference, August 8-10, Toronto, Canada; International Conference on Pharmaceutical Chemistry, August 4-6, 2016, Frankfurt, Germany; International Conference and Expo on Separation Techniques, October 31-November 2, 2016, Valencia, Spain; Winter Conference on Plasma Spectrochemistry, January 10–16, 2016, Tucson, Arizona; Characterization of Protein Therapeutics by Mass Spectrometry:  Recent Developments and Future Trends, January 21–24, 2016, Clearwater Beach, Florida; International Forum on Process Analytical Chemistry, January 24–27, 2016, Arlington, Virginia; The Pittsburgh Conference on Analytical Chemistry and Applied Spectroscopy, March 6-11, 2016, Atlanta, Georgia; Annual Practical Applications of NMR in Industry Conference, February 15-18, 2016, Houston, Texas; The Chromatographic SocietyKorean American Chromatography AssociationChinese American Chromatography Association

Track 2:- Advances in HPLC separation techniques

High Performance Liquid Chromatography (HPLC) is a non-destructive procedure for resolving a complex mixture into its individual fractions or compounds. It is based on differential migration of solutes with the solvents. The solutes in a mobile phase go through a stationary phase. Those solutes with a high affinity for the mobile phase will spend more time in this phase than the solutes that prefer the stationary phase. As the solute rise up through the stationary phase they separate. The process is called chromatographic development. The fraction with greater affinity to stationary layer travels slower and shorter distance while that with less affinity travels faster and longer.

In normal-phase chromatography, the stationary phase is polar and the mobile phase is nonpolar.

In reversed phase the stationary phase is nonpolar and the mobile phase is polar.

Flash Column Chromatography (FCC) or Flash Chromatography is a quickest and the easiest way to separate complex mixtures of compounds. It uses compressed air to push the solvent through the column. This provides better separation and reduces the amount of time required to run a column.

Ion exchange chromatography (IEC) uses an ion exchange mechanism to separate analytes based charge difference. It is performed in columns but can also be useful in planar mode. It uses a charged stationary phase to separate compounds including cations, anions, amino acids, proteins, lipids and peptides. Conventional methods use stationary phase having an ion exchange resin that carries charged functional groups that interact with oppositely charged groups of the compound to retain. IEC is commonly used to purify proteins using fast protein liquid chromatography (FPLC). Ion exchange chromatography plays an important role in the separation and purification of biomolecules. Loading samples in buffers of low ionic strength makes ion exchange chromatography an excellent purification step after HIC.

Affinity chromatography is based on selective non-covalent interaction between an analyte and sample molecules. It is highly specific, but not robust method. It is used in biochemistry in the purification of proteins bound to tags. Fusion proteins are labelled with compounds such as antigens or biotin which bind to the stationary phase specifically. Later after purification, some of these tags are removed and the pure protein is obtained. Affinity chromatography utilizes a biomolecule's affinity for a metal (Zn, Cu, Fe, etc.). Manually prepared columns are often used. Earlier affinity columns are used as a preparative step to flush out unwanted biomolecules. IMAC- Immobilized Metal Affinity Chromatography is used to separate molecules based on the relative affinity for the metal. Often these columns can be loaded with different metals to create a column with a targeted affinity.

Chiral chromatography includes the separation of stereoisomers. Enantiomers have no chemical or physical differences apart from being three-dimensional mirror images. Traditional chromatography or other separation processes are incapable of separating these. For chiral separations to happen, either the mobile phase or the stationary phase must themselves be made chiral by inducing different affinities between the analytes.

Size-exclusion chromatography (SEC) also known as gel filtration chromatography, separates molecules according to the size or more accurately according to the hydrodynamic diameter or the hydrodynamic volume. Small molecules enter the pores of the media where they are trapped and removed from the flow of the mobile phase. Mean residence time in the pores depends upon the effective size of the analyte molecules. Molecules that are larger than the average pore size of the packing are excluded and thus retention doesn’t occur; such species are the first to be eluted. It is a low-resolution chromatography technique and thus it is used for determining the tertiary structure and quaternary structure of purified proteins.

Related Conferences of Advances in HPLC Separation Techniques

International Conference and Expo on Separation Techniques, October 31-November 2, 2016, Valencia, Spain; International Conference and Exhibition on Analytical and Bioanalytical Techniques, September 29- October 1, 2016, Miami, USA; International Conference on Current Trends in Mass Spectrometry, May 9-11, 2016, Chicago, USA; World Chemistry Conference, August 8-10, Toronto, Canada; International Conference on Pharmaceutical Chemistry, August 4-6, 2016, Frankfurt, Germany; Eastern Analytical Symposium and Exposition, November 14-16, 2016, Somerset, New Jersey; Characterization of Protein Therapeutics by Mass Spectrometry:  Recent Developments and Future Trends, January 21–24, 2016, Clearwater Beach, Florida; International Forum on Process Analytical Chemistry, January 24–27, 2016, Arlington, Virginia; The Pittsburgh Conference on Analytical Chemistry and Applied Spectroscopy, March 6-11, 2016, Atlanta, Georgia; Annual Practical Applications of NMR in Industry Conference, February 15-18, 2016, Houston, Texas;Federation of Analytical Chemistry and Spectroscopy SocietiesInternational Association of Environmental Analytical ChemistryAssociation of Analytical Communities

Track 3:- Advances in HPLC instrumentations

HPLC is a popular method of analysis for natural products because of its high accuracy, precision and is not differed by the stability or the volatility of the compounds. HPLC combined with diode array detector (HPLC-DAD), mass spectrometer (HPLC-MS) have been successfully utilized for the qualitative and quantitative determination of various types of phytoconstituents like alkaloids, glycosides, tannins, tri-terpenes, flavonoids etc. HPLC methods are used readily for the determination of drug in biological fluids and pharmaceutical dosage forms. HPLC determination with spectroscopic detection is useful for routine quality control of drugs in pharmaceutical dosage forms and stability studies. Pharmaceutically important antiviral drugs (lamivudine, zidovudine), NSAIDs (acetaminophen, paracetamol), antibiotics (amoxicillin, rifampicin), CNS stimulants (amphetamine), betablockers (atenolol), preservatives (benzalkoniumchloride), Diuretics (hydrochlorthiazide), antidiabetics (metformin), anticancer (methotrexate), corticosteroids (prednisone, betamethasone, dexamethasone), anti-allergic (cetrizine), antidepressant (Nitroxazepine, milnacipran), veterinary anaesthetic (ketamine) , anti-inflammatory (sodium osagrel), secretolytic (ambroxol) and several other category drugs were successfully derived via HPLC.

HPLC columns are usually packed with pellicular or porous particles. Pellicular particles are made from polymers, or glass. Partition HPLC uses liquid bonded phase columns. The liquid stationary phase is chemically bonded to the packing material. The packing material is usually hydrolysed silica which reacts with the bond-phase coatings like siloxanes. A chromatographic detector is capable of establishing both the identity and concentration of eluting components in the mobile phase stream. A broad range of detectors are available to meet different sample requirements. Detectors respond to a particular compound only and the response is independent of mobile phase composition and the response of bulk property detectors is dependent on collective changes in composition of sample and mobile phase. Specific detectors are UV-VIS, Photo diode array, fluorescence, and mass spectroscopic detectors. Bulk Property detectors include refractive index, electrochemical and light scattering detectors.

Related Conferences of Advances in HPLC Instrumentation

International Conference and Expo on Separation Techniques, October 31-November 2, 2016, Valencia, Spain; International Conference and Exhibition on Analytical and Bioanalytical Techniques, September 29- October 1, 2016, Miami, USA; International Conference on Current Trends in Mass Spectrometry, May 9-11, 2016, Chicago, USA; World Chemistry Conference, August 8-10, Toronto, Canada; International Conference on Pharmaceutical Chemistry, August 4-6, 2016, Frankfurt, Germany; Annual North American Meeting of the Federation of Analytical Chemistry and Spectroscopy Societies, 18–23 September 2016, Minneapolis, Minnesota; International Conference on Raman Spectroscopy, August 14-19, 2016, Fortaleza, Brazil; Gordon Research Conference on Vibrational Spectroscopy, July17-22, 2016, Biddeford, Maine; International Trade Fair for Laboratory Technology, Analysis, Biotechnology and Analytica Conference, May 10-13, 2016, Munich, Germany; European Symposium on Atomic Spectrometry, March 31-April 2, 2016, Eger, Hungary;Society for Analytical ChemistryAmerican Chemical SocietyAssociation of Official Analytical Chemists

Track 4:- Hyphenated HPLC methods

The hyphenated technique is developed from the coupling of a separation technique and an on-line spectroscopic detection technology. Several remarkable improvements in hyphenated analytical methods over the last two decades have significantly broadened their applications in the analysis of biomaterials, especially natural products, pre-isolation analyses of crude extracts or fraction from various natural sources, isolation and detection of natural products, chemical fingerprinting, testing of herbal products, de-replication of natural products, and metabolomics. Rapid identification and characterization of known and new natural products directly from plant and marine sources without the necessity of isolation and purification can be achieved by various modern hyphenated techniques. Techniques like HPLC coupled to NMR (Nuclear Magnetic Resonance) or electrospray ionization tandem mass spectrometry (ESI-MS-MS) have been proven to be extremely powerful tools in natural product analysis, as they aid in the fast screening of crude natural product extracts or fractions for detailed information about metabolic profiles, with minimum quantity of material. The application of various hyphenated techniques even allows the discovery of new molecules, including complete and conclusive structure elucidation, and relative configurations as compared to time-consuming and costly isolation and purification processes. Hyphenated HPLC techniques include HPLC-MS, HPLC-ESI-MS, HPLC-IC-MS, HPLC-NMR-MS, HPLC-DAD, HPLC-CE-MS, HPLC-UV, Coupling LC and MALDI-TOF. MALDI (Matrix Assisted Laser Desorption Ionization) is a very sensitive technique for determining the mass of proteins, polymers and peptides. MALDI basically used in protein identification. MALDI sample preparation is fast and easy and therefore a primary choice in proteomics. Proteins, peptides, and polymers are fragile and tend to fragment when ionized by other ionization techniques. MALDI is attached to a time of flight analyser which measures time it takes for the molecules to travel a fixed distance. MALDI uses a short laser pulse, instead of continuous laser making it a soft ionization technique.

Related Conferences of Hyphenated HPLC Methods

International Conference and Expo on Separation Techniques, October 31-November 2, 2016, Valencia, Spain; International Conference and Exhibition on Analytical and Bioanalytical Techniques, September 29- October 1, 2016, Miami, USA; International Conference on Current Trends in Mass Spectrometry, May 9-11, 2016, Chicago, USA; World Congress on Chromatography, September 10-12, 2016, Amsterdam, Netherlands; International Conference on Pharmaceutical Chemistry, August 4-6, 2016, Frankfurt, Germany; International Symposium on Hyphenated Techniques in Chromatography and Separation Technology, 27–29 January 2016, Ghent, Belgium; The Pittsburgh Conference on Analytical Chemistry and Applied Spectroscopy, March 6-11, 2016, Atlanta, Georgia; Annual Practical Applications of NMR in Industry Conference, February 15-18, 2016, Houston, Texas; Annual Practical Applications of NMR in Industry Conference, February 15-18, 2016, Houston, Texas; The Pittsburgh Conference on Analytical Chemistry and Applied Spectroscopy, 6–11 March 2016, Atlanta, Georgia;Society for Electroanalytical ChemistryAfrican Network of Analytical ChemistsFederation of Asian Chemical Societies

Track 5:- Chip based separations

This includes a micro fabricated separation device. The availability of the fused-silica capillary marked a significant breakthrough for gas chromatography and all gas chromatographs manufactured were equipped to use fused silica capillary columns. Fused-silica capillaries have a huge contribution to the developments of other micro separation technologies like supercritical fluid chromatography. The success of one separation technique relies on sample introduction technologies, separation column and sensitive detectors that can preserve chromatographic fidelity of high resolution chromatographic peaks, as is evident from the many injectors and detectors optimized and available for open tubular GC. A particle packed column is comprised of a nano litre enrichment column and a micron or sub-micron separation column packed with suitable grade of C18. The HPLC-Chip is made from a biocompatible polyimide and the functionality of this chip is equivalent to conventional nanospray LC/MS.  Monoliths consist of a single rod of porous material with several unique features in terms of permeability and efficiency. Micro-fabricated column based on pillar-arrays were formed by arrays of nonporous silicon pillars with a diameter of approximately 4.3μm. The pillars were covalenty coated with a monolayer of hydrophobic C8-chains to enable reversed-phase LC separations.

Related Conferences of Chip Based Separations

International Conference and Expo on Separation Techniques, October 31-November 2, 2016, Valencia, Spain; International Conference and Exhibition on Analytical and Bioanalytical Techniques, September 29- October 1, 2016, Miami, USA; International Conference on Current Trends in Mass Spectrometry, May 9-11, 2016, Chicago, USA; World Chemistry Conference, August 8-10, Toronto, Canada; International Conference on Pharmaceutical Chemistry, August 4-6, 2016, Frankfurt, Germany; Annual Practical Applications of NMR in Industry Conference, February 15-18, 2016, Houston, Texas; Eastern Analytical Symposium and Exposition, November 14-16, 2016, Somerset, New Jersey; Characterization of Protein Therapeutics by Mass Spectrometry:  Recent Developments and Future Trends, January 21–24, 2016, Clearwater Beach, Florida; International Forum on Process Analytical Chemistry, January 24–27, 2016, Arlington, Virginia; The Pittsburgh Conference on Analytical Chemistry and Applied Spectroscopy, March 6-11, 2016, Atlanta, Georgia;Royal Australian Chemical InstituteBelgian Society for Mass SpectrometryCanadian Society for Chemistry

Track 6:- High efficiency and high resolution techniques

UHPLC (Ultra-HPLC) or UPLC (Ultra Performance Liquid Chromatography) is now being adopted in industrial labs, especially the pharmaceutical industry due to its high speed, high resolution and solvent saving.  A UHPLC method uses a sub-2micron column as it reduces the analysis time by 80% and save the mobile phase consumption by a huge amount compared to the conventional HPLC. In addition, the much shorter run time significantly reduces UHPLC method development scouting time. HPLC method development principles can be applied to UHPLC method development. Existing HPLC methods can be converted to UHPLC methods.

Micro and Nano HPLC ensure high levels of flow rate flexibility and reproducibility.

Hydrophilic interaction chromatography or hydrophilic interaction liquid chromatography (HILIC) is a variant of normal phase liquid chromatography that partly overlaps with other chromatographic applications such as ion chromatography and reversed phase liquid chromatography. It uses hydrophilic stationary phases with reversed-phase type eluents. Hydrophilic interaction liquid chromatography (HILIC) provides an alternative approach to effectively separate small polar compounds on polar stationary phases.

Related Conferences of High Efficiency and High Resolution Techniques

International Conference and Expo on Separation Techniques, October 31-November 2, 2016, Valencia, Spain; International Conference and Exhibition on Analytical and Bioanalytical Techniques, September 29- October 1, 2016, Miami, USA; International Conference on Current Trends in Mass Spectrometry, May 9-11, 2016, Chicago, USA; World Congress on Chromatography, September 10-12, 2016, Amsterdam, Netherlands; International Conference on Pharmaceutical Chemistry, August 4-6, 2016, Frankfurt, Germany; Annual Practical Applications of NMR in Industry Conference, February 15-18, 2016, Houston, Texas; Eastern Analytical Symposium and Exposition, November 14-16, 2016, Somerset, New Jersey; Characterization of Protein Therapeutics by Mass Spectrometry:  Recent Developments and Future Trends, January 21–24, 2016, Clearwater Beach, Florida; International Forum on Process Analytical Chemistry, January 24–27, 2016, Arlington, Virginia; The Pittsburgh Conference on Analytical Chemistry and Applied Spectroscopy, March 6-11, 2016, Atlanta, Georgia;Canadian Society for Mass SpectrometryHong Kong Society of Mass SpectrometryGerman Society for Mass Spectrometry

Track 7:- Method development

Quality can be designed to processes through systematic implementation of an optimization strategy to establish a thorough understanding of the response of the system quality to given variables, and the use of control strategies to ensure quality. The concept of method development includes modelling of the influence of values of variables on quality, design of experiments, and simplification of processes as information is collected. The extension of QbD (Quality by Design) philosophies is now applied to the development of manufacturing processes and analytical methods. The ability of a chromatographic method to successfully separate, identify and quantitate species is determined by a powerful factor called experimental design. Automation of a process is one of the keys for increasing the productivity of a research group. Scaling-up a compound separation performed on an analytical system to a preparative liquid chromatography system requires an optimization step on the analytical column. This step concerns the development of the gradient method for the isolation of the target compound with the best balance between its purity, data throughput, and analysis time.

Related Conferences of Method Development

International Conference and Expo on Separation Techniques, October 31-November 2, 2016, Valencia, Spain; International Conference and Exhibition on Analytical and Bioanalytical Techniques, September 29- October 1, 2016, Miami, USA; International Conference on Current Trends in Mass Spectrometry, May 9-11, 2016, Chicago, USA; World Congress on Chromatography, September 10-12, 2016, Amsterdam, Netherlands; International Conference on Pharmaceutical Chemistry, August 4-6, 2016, Frankfurt, Germany; Annual Practical Applications of NMR in Industry Conference, February 15-18, 2016, Houston, Texas; Eastern Analytical Symposium and Exposition, November 14-16, 2016, Somerset, New Jersey; Characterization of Protein Therapeutics by Mass Spectrometry:  Recent Developments and Future Trends, January 21–24, 2016, Clearwater Beach, Florida; International Forum on Process Analytical Chemistry, January 24–27, 2016, Arlington, Virginia; The Pittsburgh Conference on Analytical Chemistry and Applied Spectroscopy, March 6-11, 2016, Atlanta, Georgia; American Chemical Society National Spring Meeting & Exposition, March 13-17, 2016, San Diego, California;Egyptian Chemical SocietyEgyptian Society of Biochemistry and Molecular BiologyRoyal Society of Chemistry

Track 8:- Applications of HPLC

HPLC can be used in both qualitative and quantitative applications that are for both compound quantification and identification. Normal phase HPLC is rarely used now, almost all HPLC separation can be performed in reverse phase. Reverse phase HPLC (RPLC) is ineffective in for only a few separation types. HPLC is applied for molecular weight determination, in analytical chemistry, pharmaceutical and drug science, clinical sciences, food technology, and consumer products, combinatorial chemistry, polymer chemistry, environmental chemistry and green chemistry.

Related Conferences of Applications of HPLC

International Conference on Clinical Pharmacy, December 7-9, 2015, Atlanta, USA; International Conference and Exhibition on Food Processing and Technology, October 24-26, Istanbul, Turkey; International Conference on Past and Present Systems of Green Chemistry, September 19-21, 2016, Las Vegas, USA; Euro-Global Summit on Toxicology and Applied Pharmacology, October 27-29, 2016, Rome, Italy; International Conference and Exhibition on Polymer Chemistry, November 14-16, 2016, Atlanta, USA; Asia Pacific Drug Formulation and Bioavailability Congress, June 6-8, Beijing, China; The European Conference on Analytical Chemistry, September 6-10, 2016, Bordeaux, France; Annual Green Chemistry and Engineering Conference, June 14-16, Portland, OR; Prague meeting on Macromolecules Self-organization in the world of Polymers, July 10-14, 2016, Prague, CZ; Characterization of Protein Therapeutics by Mass Spectrometry:  Recent Developments and Future Trends, January 21–24, 2016, Clearwater Beach, Florida; International Conference on Clinical Chemistry and Laboratory Medicine, May 2-3, Chicago, USA;Society for Analytical Chemists of PittsburghThe Japan Society for Analytical ChemistryChromatographic Society of India

Track 9:- Biochemical applications

HPLC is a very common method for metabolomics analysis. With the invention of electrospray ionization, HPLC is coupled to mass spectroscopy. HPLC has lower chromatographic resolution, requires no derivation for polar molecules and separates molecules in the liquid phase. HPLC has the advantage of much wider range of analytes measurements with a higher sensitivity than gas chromatographic methods. Relevant to proteomics, due to the complex structure and nature of proteins, instrumentation and methods development for sample clean-up, pre-concentration, fractionation, chromatographic separation and detection becomes an immediate requirement for the identification of peptides and proteins. Latest techniques and equipment for separation and detection include nano-HPLC and multidimensional HPLC for protein and peptide separation. HPLC is considered as most reliable and most sensitive technique in genomics used to determine DNA methylation. The nucleosides and nucleotides of DNA are separated and quantified by HPLC-UV method. HPLC finds applications in glycomics and lipidomics where glycan part is cleaved either enzymatically or chemically from the target and subjected to analysis. In case of glycolipids, they can be analysed directly without separation of the lipid component. HPLC has a wide application in lipidomics to separate lipids prior to mass spectrometry. Separation can be achieved by either reverse-phase (RP) HPLC or normal-phase (NP) HPLC. Depending on the integration with the ionization source of a mass spectrometer separation of lipids using HPLC is carried out online or offline.

Related Conferences of Biochemical Applications

International Conference and Expo on Proteomics, October 24-26, Rome, Italy; International Conference on Genomics and Pharmacogenomics, September 12-14, 2016, Berlin, Germany; International Conference and Expo on Separation Techniques, October 31-November 2, 2016, Valencia, Spain; International Conference and Exhibition on Analytical and Bio analytical Techniques, September 29- October 1, 2016, Miami, USA; International Conference on Current Trends in Mass Spectrometry, May 9-11, 2016, Chicago, USA; Lipid Maps Annual Meeting 2016, May 17-18, 2016, La Jolla, CA, USA; International Symposium on Lipid Oxidation and Antioxidants; June 5-7, 2016, Porto, Portugal; Cell Symposia: Transcriptional Regulation in Development and Disease, June 26-28, 2016, Chicago, IL, USA; International Chromosome Conference, July 10-13, Foz do Iguacu, Brazil; International Conference on Proteomics & Bioinformatics, March 30-31, 2016, Istanbul, Turkey;International Association for the Advancement of High Performance Thin Layer Chromatography (HPTLC Association)Israel Society for Analytical ChemistryCalifornia Separation Science Society

Track 10:- HPLC fingerprinting in bioinformatics and computational biology

HPLC techniques are applied for purification and separation of various biological samples. The analysed samples are subjected to sequencing studies either manually or using various softwares.This is studied as Data mining and sequence analysis. HPLC is also used for characterization of various metabolites.

Related Conferences of HPLC Fingerprinting in Bioinformatics and Computational Biology

International Conference on Bioinformatics, March 29-30, Valencia, Spain; International Conference and Expo on Proteomics, October 24-26, Rome, Italy; International Conference on Genomics and Pharmacogenomics, September 12-14, 2016, Berlin, Germany; International Conference and Expo on Separation Techniques, October 31-November 2, 2016, Valencia, Spain; International Conference on Current Trends in Mass Spectrometry, May 9-11, 2016, Chicago, USA; International Conference on Bioinformatics Models, Methods and Algorithms; International Symposium on Lipid Oxidation and Antioxidants; June 5-7, 2016, Porto, Portugal; Cell Symposia: Transcriptional Regulation in Development and Disease, June 26-28, 2016, Chicago, IL, USA; International Chromosome Conference, July 10-13, Foz do Iguacu, Brazil; International Conference on Proteomics & Bioinformatics, March 30-31, 2016, Istanbul, Turkey;Royal Society of ChemistryIndian Society for Electroanalytical ChemistryThe Japan Society for Analytical Chemistry

Track 11:- Recent advances in HPLC

The HPLC methodology applied to the analysis of biological samples makes it possible for the identification of many metabolites. Samples from two human embryos culture medium were analysed by high-pressure liquid chromatography–mass spectrometry (HPLC–MS). They work on the principle that many microorganisms have their own unique mass spectral signature based on the particular proteins and peptides that are present in the cells. Identification of unknown peaks in gas chromatography (GC-MS)-based discovery metabolomics is challenging, and remains necessary to permit discovery of novel or unexpected metabolites that may allergic diseases  processes and/or further our understanding of how genotypes relate to phenotypes. Here, we introduce two new technologies and advances in pharmaceutical analytical methods that can facilitate the identification of unknown peaks.

Related Conferences of Chip Based Separations

International Conference and Expo on Separation Techniques, October 31-November 2, 2016, Valencia, Spain; International Conference and Exhibition on Analytical and Bioanalytical Techniques, September 29- October 1, 2016, Miami, USA; International Conference on Current Trends in Mass Spectrometry, May 9-11, 2016, Chicago, USA; World Chemistry Conference, August 8-10, Toronto, Canada; International Conference on Pharmaceutical Chemistry, August 4-6, 2016, Frankfurt, Germany; Annual Practical Applications of NMR in Industry Conference, February 15-18, 2016, Houston, Texas; Eastern Analytical Symposium and Exposition, November 14-16, 2016, Somerset, New Jersey; Characterization of Protein Therapeutics by Mass Spectrometry:  Recent Developments and Future Trends, January 21–24, 2016, Clearwater Beach, Florida; International Forum on Process Analytical Chemistry, January 24–27, 2016, Arlington, Virginia; The Pittsburgh Conference on Analytical Chemistry and Applied Spectroscopy, March 6-11, 2016, Atlanta, Georgia;Society for Analytical Chemists of PittsburghAmerican Chemical SocietyThe Chromatographic Society

Track 12:- Market growth of HPLC

The HPLC industry is showing rapid growth, with value expected to hit 228 billion dollars up from 164 US billion dollars by 2016, with an annual growth of nearly 7%, according to a recent industrial marketing research report. Geographically, global separation technique market has been segmented into four areas namely, North America, some parts of European region, Asia-Pacific and Rest of the World.

Related Conference of Market Growth of Chromatography

International Conference and exhibition on Materials Chemistry  March 31-Apr 1, 2016 Valencia, Spain; 3rd International Conference on Past and Present Research Systems of Green Chemistry, September 19-21, 2016, Las Vegas, USA; International Conference on Organic Chemistry, August 11-13, 2016, Las Vegas, USA; International Conference and Exhibition on Polymer Chemistry, November 14-16, 2016, Atlanta, USA; International Conference on Petroleum Engineering, September 12-14, 2016, Phoenix, USA; Asia Pacific Mass Spectrometry Congress, October 10-12, 2016, Kuala Lumpur, Malaysia; 2nd International Conference on Current Trends in Mass Spectrometry, May 09-11, 2016, Chicago, USA; 7th International Conference and Exhibition on Analytical & Bio-analytical Techniques, September 29-October 01, 2016 Miami, USA; World Chemistry Conference August 08-10, 2016 Toronto, Canada; 2nd International Conference and Expo on Separation Techniques Oct 31- Nov 02, 2016 Valencia, Spain; 9th National Meeting on Chromatography, January 5-9, 2016 Lisbon, Portugal; The 5th Annual SLAS Conference and Exhibition, January 23-27, 2016, San Diego, USA; Pittcon Conference & Expo, March 6-10, 2016 Atlanta, USA; Experimental Nuclear Magnetic Resonance Conference, April 10-15, 2016Pittsburgh, United States; 64th ASMS Conference on Mass Spectrometry and Allied Topics, June 5-9 2016 San Antonion, United States; 29th International Symposium on Preparative and Process Chromatography, July 17-20, 2016 Philadelphia, USA; 44th International Symposium on High Performance Liquid Phase Separations and Related Techniques, June 19-24, 2016 San Francisco, USA; 21st International Mass Spectrometry Conference, August 20-26, 2016Toronto, Canada; 31st International Symposium on Chromatography, August 28-September 1, 2016 Cork, Ireland; Asia Pacific Mass Spectrometry Congress October, 10-12, 2016 Kuala Lumpur, Malaysia; Chemistry Conferences Europe June 16-18, 2016 Rome, Italy;Association of Analytical CommunitiesChinese American Chromatography AssociationInternational Association of Environmental Analytical Chemistry

HPLC 2017 Congress organizing committee invites analytical expertise, researchers, professors, scientific communities, delegates, students, business professionals and executives to attend the “International Conference and Exhibition on Advances in HPLC and Chromatography Techniques” which is to be held during March 20-22, 2017 at Kuala Lumpur, Malaysia.

In the light of this theme, the conference series aims to provide a forum for international researchers from various areas of analytical chemistry, pharmacy, pharmacology, bioinformatics and other life science groups by providing a platform for critical analysis of new data, and to share latest cutting-edge research findings and results about all aspects regarding advances in HPLC and Chromatography techniques.

The conference provides a platform to detail the research works of analytical expertise from various scientific backgrounds and the same can be perceived by young researchers and students. The conference mainly aims to promulgate knowledge on chromatography and unveil the advances in HPLC techniques. Both life sciences and chemical sciences need analytical techniques in course of research work and therefore HPLC Congress 2017 would be a perfect venue to share and develop knowledge on key analytical tools.

Target Audience:

Analytical experts in chromatography

Research Heads from Research Centres

Leads from Pharmacy and Chemical Industries

Industrial expertise working with various novel solid and liquid columns

Marketing teams of Industries with novel products to show case at the conference

Directors and Professors from Universities and Institutions

Post-doctoral and PhD students working on analytical method development

Theoretical scientists working on deriving analytical hypotheses

Relevant Graduate and Post graduate students

The global life science and chemical instrumentation market is expected to reach $48.84 billion by 2019, at CAGR of 6.9%. Technological advancements in the analytical instruments, rising government spending on pharmaceutical R&D in emerging nations, increasing public and private life science research and development expenditure, growing food safety concerns, and progressing drug discovery and clinical diagnostics field are the key factors driving the growth of life science and chemical instrumentation market. Furthermore, growing proteomics market, rising environmental safety concerns, and increasing demand of analytical instruments from applied markets are some of the key factors offering new growth opportunities for the market players. The Europe Liquid Chromatography Reagents market is estimated to grow at a CAGR of 9.9% from 2014 to 2019. The market growth is fuelled by extensive use of chromatographic techniques in every minute analysis.

OMICS International organizes 1000+ Global events  every year across  the globe with support from 1000+ more scientific societies and Publishes 500 Open access journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

With great keenness, we look forward to seeing you at HPLC Congress 2017.

HPLC (High Performance Liquid Chromatography) is the most popular analytical technique for a wide variety of samples from typical biological compounds to those in polluted environment. HPLC is unique because of its features like pressure stable column, constant and high linear velocity of mobile phase and most important densely packed column with reduced particle size, less than 10µm (less than 2µm in UHPLC) .

Since its invention, chromatography has become a key tool for separation, identification, purification and quantification of several compounds. Chromatography is under constant development owing to the growing research needs in academic and industrial levels. This has led to the combination of HPLC with other spectroscopic techniques like NMR, MS, GC, IC, SE and MALDI. Hyphenated HPLC techniques are now more investigated and used to combine the efficiency of more than one technique. The particle size in a chromatography column is now being reduced to as much as sub-micro and sub-nano thus increasing the height of research.

Why in Kuala Lumpur?

The presence of universities, hospitals, legal/accounting and other professional services, together with key installations of the U.S. make it favorite destination for scientific meetings 

The Kuala Lumpur economy is dominated by four major sectors: oil/gas and related activities, tourism, the port and ship/boat building, and aerospace manufacturing. Boasting attractions such as its magnetic French Quarter,  Kuala Lumpur has a history of solid tourist trade. In a city with more than 5 million visitors annually, the hospitality business supplies more than 66,000 jobs in the service sector such as accommodations and restaurants. In 2004, tourists spent $4.9 billion in Kuala Lumpur.

Market Summary

The enormous market of chromatography instrumentation includes chromatography systems like TLC, HPLC, UPLC etc. and chromatography consumables like filters, columns, solvents etc. The international life science and chemical instrumentation market is expected to increase at CAGR of 6.9% reaching $48.84 billion by 2019. Increasing government investments in pharmaceutical R&D in emerging nations, technological advancements in the analytical instruments, growing food safety concerns, increasing life science research and development expenditure, and progressing drug discovery and clinical diagnostics field are the key factors driving the growth of life science and chemical instrumentation market. Additionally, the key factors offering new growth opportunities for the market players include rising environmental safety concerns, growing proteomics market and increasing demand of analytical instruments from applied markets. The Europe Liquid Chromatography Reagents market is determined to grow at a CAGR of 9.9% from 2014 to 2019. The market growth is stimulated by extensive use of chromatographic techniques in every minute analysis.

Why to attend HPLC Congress 2017?

HPLC Congress 2017 is a cluster conference that provides a platform to detail the research works of analytical expertise from various scientific backgrounds and the same can be perceived by young researchers and students. The conference mainly aims to promulgate knowledge on chromatography and unveil the advances in HPLC techniques. Both life sciences and chemical sciences need analytical techniques in course of research work and therefore HPLC Congress 2017 would be a perfect venue to share and develop knowledge on key analytical tools. The “International Conference and Exhibition on Advances in HPLC and Chromatography Techniques” caters the exhibition of products and services from various commercial and non-commercial organizations thus interlinking academia and business.

Global HPLC and Chromatography Associations

Chromsoc- The Chromatographic Society

Association of Separation Scientists and Technologists

Society of Analytical Psychology

Chinese American Chromatography Association

American Chemical society

Analytical chemistry

Separation Science and Chemical Analysis

Chromatographic Society of India

Federation of Asian Chemical Societies

European Society for Separation Science

Hungarian society of Separation Sciences

CASSS- An international Separation Science Society

Chinese Society of Chromatography

Swiss Chemical Society

HPLC Congress 2016

We delightedly thank our wonderful Speakers, Conference Attendees Students, Media Partners, associations and Sponsors for making HPLC Congress 2016 the best ever!

International conference and Exhibition on Advances in HPLC and Chromatography Techniques, hosted by the OMICS International was held during March 14-15, 2016 London, UK at Crowne Plaza Heathrow London Airport Hotel with the theme “Exploring the Widespread Applications of Chromatography and Unveiling Advances in HPLC” which got magnificent response. With the support and guidance of Organizing Committee Members and Editorial Board Members and astonishing presentations of all participants, this prominent event became more impressive.

OMICS International expresses its gratitude to the conference Moderator, Dr Kal Karim for taking up the responsibility to coordinate during the sessions. We are indebted to your support.

OMICS International would like to convey a great appreciation to following Honorable guests and Keynote speakers.

Boguslaw Buszweski, Nicolaus Copernicus University, Poland

Jacob de Boer, VU University, The Netherlands

Dusan Berek, Slovak Academy of Sciences, Slovakia

Kal Karim, University of Leicester, UK

Sergey A Piletsky, University of Leicester, UK

Hiroki Kuwano, Tohoku University, Japan

OMICS International extends its gratitude towards Poster Judge Dr Hiroki Kuwano. We also congratulate the Best Poster awardee Ms Ann Gogolashvili, Tbilisi State University, Georgia for her outstanding performance in the field of HPLC and Chromatography and appreciate all the participants who put their efforts in poster presentations and sincerely wish them success in future endeavours.

Workshops and Symposium for HPLC Congress 2016

Advanced Liquid Chromatography Methods for Comprehensive Molecular Characterization of Synthetic Polymers

Organizers: Dusan Berek, Slovak Academy of Sciences, Slovakia

OMICS International offers its heartfelt thanks to Media Partners and Collaborators (Medical News Today, Chemie.de, Bentham Science, Chemical Search International and European Pharmaceutical Review) and other eminent personalities who supported the conference by facilitating the discussion forums. OMICS International also took privilege to felicitate the Keynote Speakers, Organizing Committee Members, Chairs and Exhibitors who supported this event.

With sustainable excitement for grand success of HPLC Congress 2016, OMICS International is glad to announce next annual meeting “3rd International Conference and Exhibition on Advances in HPLC and Chromatography Techniques” to be held during March 20-22, 2017 with the theme “A focus on Innovative Techniques in HPLC and Chromatography”

We hope your favourable support will make next annual meeting also one more grand successful event.

For HPLC Congress 2016 ps: http://hplc.conferenceseries.com/2016

 


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